Genotypic Variation for High Temperature Tolerance in Relation to Carbon Partitioning and Grain Sink Activity in Wheat

Bavita Asthir, Puneet Kaur Rai, Navtej S. Bains, Virinder S. Sohu

Department of Biochemistry, Punjab Agricultural University, Ludhiana, India.
Department of Plant Breeding and Genetics, Punjab Agricultural University, Ludhiana, India.

DOI: 10.4236/ajps.2012.33046                

Abstract

Effect of high temperature tolerance in relation to carbon partitioning and grain sink activity was studied in ten genotypes of wheat (viz C 306, PBW 343, PBW 502, PBW 509, PBW 521, PBW 522, PBW 550, Inqalab, WL 711 and Sonalika) under normal (November, 25.6°C during grain filling) and late planting conditions (December, 29.4°C). Contents of reducing sugars first increased till actively metabolizing stage i.e. 14 days after anthesis (DAA) in parallel with the activity of acid invertase and thereafter declined. Significant reduction in total free sugars and sucrose content was observed during grain development. Acid invertase activity got induced especially in PBW 343 and C 306 under late sown conditions, whereas sucrose synthase, total amylase activities got repressed. Sucrose synthase was significantly high at all stages and it predominated over invertases. Neutral invertase and total amylase showed peak activity of one week earlier in the late sown crop as compared to normal sown crop. A premature cessation of starch deposition occurred in cvs PBW 521, PBW 522 under late sown conditions due to shortening of grain filling period. Our results suggest that decline in sugar content in spite of high sucrolytic enzymes may be correlated to more utilization of assimilates over production/translocation for grain sink activity under high temperature influences. Knowledge of the mechanisms that underlie carbohydrate partitioning in diverse genotypes could be exploited for generating germplasm that can tolerate high temperature.

Keywords

Carbohydrate Metabolism; Genotypes; Grains; Heat Stress; Sucrose; Starch; Triticum aestivum L.

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	H. Zhao, T. Dai, Q. Jing, D. Jiang and W. Cao, “Leaf Senescence and Grain Filling Affects by Post Anthesis High Temperatures in Two Different Wheat Cultivars,” Plant Growth Regulation, Vol. 51, No. 2, 2007, pp. 149-158. doi:10.1007/s10725-006-9157-8
[2]	G. K McDonald, B. G Sutton and F. W Ellison, “The Effect of Time of Sowing on the Grain Yield of Irrigated Wheat in the Naomi Valley, New South Wales,” Australian Journal of Agricultural Research, Vol. 34, No. 3, 1983, pp. 229-240. doi:10.1071/AR9830229
[3]	N. B Singh, Y. P Singh and V. P. N Singh, “Variation in Physiological Traits in Promising Wheat Varieties under Late Sown Condition,” Indian Journal of Plant Physiology, Vol. 10, No. 2, 2005, pp. 171-175.
[4]	A. Blum and B. Sinmena, “Wheat Seed Endosperm Utilization under Heat Stress and Its Relationship to Thermotolerance in Autotrophic Plants,” Field Crop Research, Vol. 37, No. 3, 1994, pp. 185-191. doi:10.1016/0378-4290(94)90097-3
[5]	B. Asthir, A. S. Basra and S. K Batta, “Fluoride-Induced Alteration of Carbon and Nitrogen Metabolism in Developing Wheat Grains,” Biologia Plantarum, Vol. 41, No. 2, 1998, pp. 287-292. doi:10.1023/A:1001895420374
[6]	S. S Bhullar and C. F. Jenner, “Effects of a Brief Episode of Elevated Temperature on Grain Filling in Winter Wheat Ears Cultured on Solutions of Sucrose,” Australian Journal of Plant Physiology, Vol. 13, No. 5, 1986, pp. 617-626. doi:10.1071/PP9860617
[7]	N. Cheikh and R. J. Jones, “Heat Stress Effects on Sink Activity of Developing Maize Kernels Grown in Vitro,” Plant Physiology, Vol. 95, No. 1, 1995, pp. 59-66. doi:10.1111/j.1399-3054.1995.tb00808.x
[8]	M. A. B. Wallwork, S. J. Logue, L .C. MacLeod and C. F. Jenner, “Effects of High Temperature during Grain Filling on Starch Synthesis in the Developing Barley Grain,” Australian Journal of Plant Physiology, Vol. 25, No. 2, 1998, pp. 173-181. doi:10.1071/PP97084
[9]	L. C. Ho, “Metabolism and Compartmentation of Imported Sugars in Sink Organs in Relation to Sink Strength,” Annual Rev Plant Physiology Molecular Biology, Vol. 39, 1988, pp. 355-378. doi:10.1146/annurev.pp.39.060188.002035
[10]	J. Yang, J. Zhang, Z. Wang, G. Xu and Q. Zhu, “Activities of Key Enzymes in Sucrose to Starch Conversion in Wheat Grains Subjected to Water Deficit during Grain Filling,” Plant Physiology, Vol. 135, No. 3, 2004, pp. 1621-1629. doi:10.1104/pp.104.041038
[11]	C. A. Lovell, P. T. Tomlinson, K. E. Koch, “Sucrose Metabolizing Enzymes in Transport Tissues and Adjacent Sink Structures in Developing Citrus Fruit,” Plant Physiology, Vol. 90, No. 4, 1989, pp. 1394-1402. doi:10.1104/pp.90.4.1394
[12]	D. P. Xu, S. S. Sung and C. C. Black, “Sucrose Metabolism in Lema Bean Seeds,” Plant Physiology, Vol. 89, No. 4, 1989, pp. 1106-1116. doi:10.1104/pp.89.4.1106
[13]	L. I Tian, L. Qi-Hua, R. Ohsugi, T. Yamagishi, D. H Sasaki, “Effect of High Temperature on Sucrose Content and Sucrose Cleaving Enzyme Activity in Rice Grain during the Filling Stage,” Rice Science, Vol. 13, No. 3, 2006, pp. 205-210.
[14]	A. Wahid, S. Gelani, M. Ashraf and M. R. Foolad, “Heat Tolerance in Plants: An Overview,” Environmental and Experimental Botany, Vol. 61, No. 3, 2007, pp. 199-223. doi:10.1016/j.envexpbot.2007.05.011
[15]	H. T. Buck, J. E. Nisi and N. Salomon (Eds.), “Wheat Production in Stressed Environments,” Springer, Amsterdam, 2007, pp. 365-371.
[16]	B. Venkateswarlu and R. M. Visperas, “Sucrose-Sink Relationships in Crop Plants,” International Rice Research Paper Series, Vol. 125, 1987, pp. 1-19.
[17]	J. S. Liang, J. H. Zhang and X. Z. Cao, “Grain Sink Strength May Be Related to the Poor Grain Filling of indica-japonica Rice (Oryza sativa) Hybrids,” Plant Physiology, Vol. 112, No. 4, 2001, pp. 470-477. doi:10.1034/j.1399-3054.2001.1120403.x
[18]	U. Wobus, N. Sreenivasulu, L. Borisjuk, H. Rolletschek, R. Panitz, S. Gubatz and W. Weschke, “Molecular Physiology and Genomics of Developing Barley Grains,” Recent Research Development in Plant Molecular Biology, Vol. 2, 2005, pp. 1-29.
[19]	C. F. Jenner, “An Investigation of the Association between the Hydrolysis of Sucrose and Its Absorption by Grains of Wheat,” Australian Journal of Plant Physiology, Vol. 1, No. 3, 1974, pp. 319-329. doi:10.1071/PP9740319
[20]	B. Asthir, A. S. Basra and S. K. Batta, “Differential Response of Carbon and Nitrogen Metabolism to Fluoride Application in Fruiting Structures of Chickpea,” Acta Physiologia Plantarum, Vol. 21, No. 1, 1999, pp. 67-73. doi:10.1007/s11738-999-0029-5
[21]	O. H Lowry, N. J Rosebrough, A. L. Farr and R. J. Ranadall, “Protein Measurement with the Folin Phenol Reagent,” Journal of Biological Chemistry, Vol. 193, No. 1, 1951, pp. 26-75.
[22]	N. Nelson, “A Photometric Adaptation of Somogyi Method for the Determination of Glucose,” Journal of Biological Chemistry, Vol. 153, No. 2, 1944, pp. 375-380.
[23]	E. Acevedo, M. Nachit and G. Ferrara Ortiz, “Effects of Heat Stress on Wheat and Possible Selection Tools for Use in Breeding for Tolerance,” In: D. A. Saunders, Ed., Wheat for the Nontraditional Warm Areas, Centro Internacional de Mejoramiento de Maiz y Trigo, Mexico City, 1991, pp. 401-421.
[24]	F. Rook, S. A Hadingham, Y. Li and M. W. Bevan, “Sugar and ABA Response Pathways and the Control of Gene Expression,” Plant Cell and Environment, Vol. 29, No. 3, 2006, pp. 426-434. doi:10.1111/j.1365-3040.2005.01477.x
[25]	S. C. B. R. Jose, E. V. R. Von Pinho and M. A. G. S. Dias, “Sugars and Tolerance to High Drying Temperature in Corn Seeds,” Rev Bras SementesI, Vol. 28, 2006, pp. 60-68.
[26]	E. M. Dale and T. L. Housely, “Sucrose Synthase Activity in Developing Wheat Endosperms Differing in Maximum Weight,” Plant Physiology, Vol. 82, No. 1, 1986, pp. 7-10. doi:10.1104/pp.82.1.7
[27]	S. Bala, B. Asthir and N. S. Bains, “High Temperature Response Leads to Altered Membrane Permeability in Conjunction with Carbon Utilization in Wheat,” Seed Science & Biotechnology, Vol. 4, No. 1, 2010, pp. 10-14.
[28]	Q. Zhou, D. Jiang, T. Dai, Q. Jing and W. Cao, “Regulation of Starch and Protein Synthesis in Wheat Grains by Feeding Sucrose and Glutamine to Detached Ears Cultured in Vitro,” Plant Growth Regulation, Vol. 48, No. 3, 2006, pp. 247-259
[29]	S. S. Sung, W. J. Sheih, D. R. Geigre and C. C. Black, “Growth, Sucrose Synthase and Invertase Activities of Developing Phaseolus vulgaris L. Fruits,” Plant Cell & Environment, Vol. 17, No. 4, 1994, pp. 419-426. doi:10.1111/j.1365-3040.1994.tb00310.x
[30]	S. C. Huber and T. Akazawa, “A Novel Sucrose Synthase Pathway for Sucrose Degradation in Cultured Sycamore Cell,” Plant Physiology, Vol. 81, No. 4, 1986, pp. 1008-1013. doi:10.1104/pp.81.4.1008
[31]	G. Avigad and P. M Dey, “Carbohydrate Metabolism: Storage Carbohydrates,” In: P. M. Dey and J. B. Harborne, Eds., Plant Biochemistry, Academic Press, Cambridge, 2000, pp. 143-203
[32]	C. M. Duffus and R. Rosie, “Starch Hydrolysing Enzymes in the Developing Barley Grain,” Planta, Vol. 109, No. 2, 1973, pp. 153-160. doi:10.1007/BF00386123
[33]	C. Y, Tsai, F. Salamini and O. E. Nelson, “Enzymes of Carbohydrate Metabolism in Developing Endosperm of Maize,” Plant Physiology, Vol. 4, No. 2, 1970, pp. 299-306. doi:10.1104/pp.46.2.299
[34]	C. P. P Ricardo and T. après, “Invertase Activity during the Development of Carrot Roots,” Phytochemistry, Vol. 9, No. 2, 1970, pp. 239-247. doi:10.1016/S0031-9422(00)85130-4
[35]	D. A. Morris and E. D. Arthur, “Invertase Activity, Carbohydrate Metabolism and Cell Expansion in the Stem of Phaseolus vulgaris L.,” Journal of Experimental Botany, Vol. 36, No. 4, 1985, pp. 623-633. doi:10.1093/jxb/36.4.623
[36]	S. Alka and R. Khanna-Chopra, “Influence of Temperature on Germination and Seedling Growth and Its Relationship with Amylase Activity and Respiration in Wheat Varieties Differing in Temperature Tolerance,” Indian Journal of Experimental Biololgy, Vol. 33, No. 11, 1995, pp.775-779.
[37]	P. Prakash, P. Sharma-Natu and M. C. Ghildiyal, “High Temperature Effect on Starch Synthase Activity in Relation to Grain Growth in Wheat Cultivars,” Indian Journal of Plant Physiology, Vol. 8, 2003, pp. 390-398.
[38]	C. M. Duffus and M. P. Cochrane, “Grain Structure and Composition,” In: P. R. Shewry Ed., Barley: Genetics, Molecular Biology and Technology, C.A.B. International, London, 1992, pp. 219-317.
[39]	J. Yang, J. Zhang, Z. Wang, G. Xu and Q. Zhu, “Activities of Key Enzymes in Sucrose to Starch Conversion in Wheat Grains Subjected to Water Deficit during Grain Filling,” Plant Physiology, Vol. 135, No. 3, 2004, pp. 1621-1629. doi:10.1104/pp.104.041038