Biography

Prof. Roberto Fernández-Lafuente

Consejo Superior de Investigaciones Científicas, Spain
Professor


Email: rfl@icp.csic.es


Qualifications

1993-1996 Post doctorate, University Collegue London, United Kingdom


Publications (Selected)

  1. Gloria Fernandez-Lorente, Cesar Godoy, Adriano A. Mendes, Fernando Lopez-Gallego, Valeria Grazu, Blanca de las Rivas, Jose M. Palomo, Juan Hermoso, Roberto Fernandez-Lafuente* and Jose M. Guisan* 2008 SOLID-PHASE CHEMICAL AMINATION OF A LIPASE FROM Bacillus thermocatenulatus TO IMPROVE ITS STABILIZATION VIA COVALENT IMMOBILIZATION ON HIGHLY ACTIVATED GLYOXYL-AGAROSE. Biomacromolecules 9; 2553-2561.
  2. Marco Filice, Jose M Palomo, Paolo Bonomi, Teodora Bavaro, Roberto Fernandez-Lafuente, Jose M Guisan and Marco Terreni,2008 Preparation of linear oligosaccharides by a simple monoprotective chemo-enzymatic approach. Tetrahedron. 64, 9286-9292.
  3. Samia Kaddour, Fernando López-Gallego, Tahar Sadoun, Roberto Fernandez-Lafuente* and Jose M. Guisan*. (2008) PREPARATION OF AN IMMOBILIZED-STABILIZED CATALASE DERIVATIVE FROM Aspergillus niger HAVING ITS MULTIMERIC STRUCTURE STABILIZED: THE EFFECT OF Zn2+ON ENZYME STABILITY. J. Mol. Cat. B: Enzym. 55, 142–145.
  4. Adriano A. Mendes, Dasiana S. Rodrigues, Marco Filice, Roberto Fernandez-Lafuente, Jose M. Guisan and Jose M. Palomo. 2008. Regioselectivity alteration of lipases by immobilization in the monohydrolysis of per-O-acetylated 1-O-substituted-β-glucopyranosides. Tetrahedron 64, 10721-10727.
  5. K. Rose, S. Dzyadevych, R. Fernández-Lafuente, N. Jaffrezic, G. Kuncová, V. Matějec, P. Scully 2008 Hybrid Coatings as Transducers in Optical Biosensors J. Coating. Technol. Res. 5, 491 – 496.
  6. Cesar Carrasco-López, Cesar Godoy, Blanca de las Rivas, Gloria Fernandez-Lorente, Jose M. Palomo, José M. Guisán, Roberto Fernández-Lafuente, Martín Martínez-Ripoll and Juan A. Hermoso 2008 Crystallization of a bacterial thermoalkalophilic lipase BTL2 from Bacillus thermocatenolatus. Acta Cryst. F, 64, 1043–1045.
  7. Zaida Cabrera, Gloria Fernandez-Lorente, Jose M. Palomo, Jose M. Guisan*, Roberto Fernandez-Lafuente*. 2008 Asymmetric hydrolysis of dimethyl 3-phenylglutarate catalyzed by Lecitase Ultra®. Effect of the immobilization protocol on its catalytic properties. Enzyme Microb. Technol. 43, 531–536.
  8. Marco Filice, Teodora Bavaro, Roberto Fernandez-Lafuente, Massimo Pregnolato, Jose M Guisan, Jose M Palomo and Marco Terreni. 2009. Chemo-Biocatalytic Regioselective One Pot Synthesis of Different Deprotected Monosaccharides Catalysis today. 140, 11-18.
  9. Zaida Cabrera, Gloria Fernandez-Lorente, Roberto Fernandez-Lafuente, Jose M. Palomo and Jose M. Guisan. 2009 Enhancement of Novozym-435 catalytic properties by physical or chemical modification. Proc. Biochem. 44, 226-231.
  10. Juan M. Bolivar, Cesar Mateo, Javier Rocha-Martin, Felipe Cava, Jose Berenguer, Roberto Fernandez-Lafuente* and Jose M. Guisan*. 2009 THE ADSORPTION OF MULTIMERIC ENZYMES ON VERY LOWLY ACTIVATED SUPPORTS INVOLVES MORE ENZYME SUBUNITS: STABILIZATION OF GLUTAMATE DEHYDROGENASE FROM Thermus thermophilus BY IMMOBILIZATION ON HETEROFUNCTIONAL SUPPORTS.Enzyme Microb. Technol. 44, 139-144.
  11. Zaida Cabrera, Gloria Fernandez-Lorente, Roberto Fernandez-Lafuente, Jose M. Palomo and Jose M. Guisan. 2009, Novozym 435 displays very different selectivity compared toCandida antarctica Blipase adsorbed on standard hydrophobic supports J. Mol. Cat. B: Enzym. 57, 171-176.
  12. Aline G. Cunha, Gloria Fernández-Lorente, Melissa L. E. Gutarra, Juliana V. Bevilaqua, Rodrigo V. Almeida, Lúcia M. C. Paiva, Roberto Fernández-Lafuente, Jose M. Guisán, Denise M. G. Freire. 2009. Separation and immobilization of lipase from Penicillium simplicissimum by selective adsorption on hydrophobic supports Applied Biochemistry and Biotechnology, 156, 563–575.
  13. Dasciana S. Rodrigues, Adriano A. Mendes, Marco Filice, Roberto Fernandez-Lafuente, Jose M. Guisan and Jose M. Palomo. 2009 Different derivatives of a lipase display different regioselectivity in the monohydrolysis of per-O-acetylated 1-O-substituted-β-galactopyranosides. J. Mol. Cat. B: Enzym. 58, 36-40.
  14. Pilar Batalla, Cesar Mateo, Valeria Grazu, Roberto Fernandez-Lafuente* and Jose M. Guisan*. 2009 IMMOBILIZATION OF ANTIBODIES THROUGH THE SURFACE REGIONS HAVING THE HIGHEST DENSITY IN LYSINE GROUPS ON FINALLY INERT SUPPORT SURFACES. Proc. Biochem 44, 365-368.
  15. Juan M. Bolivar, Javier Rocha-Martin, Cesar Mateo, Felipe Cava, Jose Berenguer, Daniel Vega, Roberto Fernandez-Lafuente* and Jose M. Guisan*. 2009. Purification and stabilization of a glutamate dehygrogenase from Thermus thermophilus via oriented multisubunit plus multipoint covalent immobilization. J. Mol. Cat B: Enzym. 58, 158-163.
  16. César Carrasco-López, César Godoy, Blanca de las Rivas, Gloria Fernández-Lorente, José M. Palomo, José M. Guisán, Roberto Fernández-Lafuente, Martín Martínez-Ripoll, and Juan A. Hermoso 2009. Activation of bacterial thermoalkalophilic lipases is spurred by dramatic structural rearrangements J. Biol. Chem. 284, 4365-4372.
  17. César Carrasco-López, César Godoy, Blanca de las Rivas, Gloria Fernández-Lorente, José M. Palomo, José M. Guisán, Roberto Fernández-Lafuente, Martín Martínez-Ripoll, and Juan A. Hermoso 2009 Cubierta del numero 7 del volumen 284 de la revista J. Biol. Chem.
  18. Oscar Romero, José Vergara, Roberto Fernández-Lafuente, José Manuel Guisán, Andrés Illanes, Lorena Wilson. 2009. Simple strategy of reactivation of a partially inactivated penicillin acylase biocatalyst in organic solvent and its impact in the synthesis of β-lactam antibiotics. Biotechnol. Bioeng. 103, 472-479.
  19. Rafael C. Rodrigues, Juan M. Bolivar, Armand Palau-Ors, Giandra Volpato, Marco A. Z. Ayub, Roberto Fernandez-Lafuente* and Jose M. Guisan* 2009. Positive effects of the multipoint covalent immobilization in the reactivation of partially inactivated derivatives of lipasefrom Thermomyces lanuginosus. Enzyme Microb Technol. 44, 386-393.
  20. Rafael C. Rodrigues, Cesar A. Godoy, Marco Filice, Juan M. Bolivar, Armand Palau-Ors, Jesus M. Garcia-Vargas, Oscar Romero, Lorena Wilson, Marco A. Z. Ayub, Roberto Fernandez-Lafuente* and Jose M. Guisan*. 2009. Reactivation of covalently immobilized lipase from Thermomyces lanuginosus. Process Biochem . 44, 641-646.
  21. Juan M. Bolivar, Javier Rocha-Martin, Cesar Mateo, Felipe Cava,Jose Berenguer, Roberto Fernandez-Lafuente* and Jose M. Guisan*. 2009 COATING OF SOLUBLE AND IMMOBILIZED ENZYMES WITH IONIC POLYMERS: FULL STABILIZATION OF THE QUATERNARY STRUCTURE OF MULTIMERIC ENZYMES. Biomacromolecules. 10, 742–747.
  22. Juan M. Bolivar, Cesar Mateo, Cesar Godoy, Benevides C.C. Pessela, Dasciana S. Rodrigues,Raquel L. C. Giordano Roberto Fernandez-Lafuente* and Jose M. Guisan* 2009. The Co-operative Effect of Physical and Covalent Protein Adsorption on Heterofunctional Supports. Process. Biochem. 44, 757–763.
  23. Rafael C. Rodrigues, Cesar Godoy, Giandra Volpato, Marco A. Z. Ayub, Roberto Fernandez-Lafuente* and Jose M. Guisan*. 2009 Immobilization-stabilization of the lipase from Thermomyces lanuginosus: the critical role of the chemical amination. Process Biochem. 44, 963-968.
  24. Javier Rocha-Martin, Daniel E. Vega, Zaida Cabrera, Juan M. Bolivar, Roberto Fernandez-Lafuente*, Jose Berenguer* and Jose M. Guisan*2009. Purification, immobilization and stabilization of a highly enantioselective alcohol dehydrogenase from Thermus thermophilus HB27 cloned in E. coli. Process Biochem. 44, 1004-1012.
  25. Roberto Fernandez-Lafuente Stabilization of multimeric enzymes: strategies to prevent sub-unit dissociation 2009. Enzyme Microb. Technol. 45, 405–418.
  26. Rafael C. Rodrigues, Juan M. Bolivar, Giandra Volpato, Marco Filice, Cesar Godoy, Roberto Fernandez-Lafuente* and Jose M. Guisan* 2009. Improved reactivation of immobilized-stabilized lipase fromThermomyces lanuginosus by its coating with highly hydrophilic polymers. J. Biotechnol. 144, 113–119.
  27. Roberto Fernandez-Lafuente. LIPASE FROM Thermomyces lanuginosus. USES AND PROSPECTS AS AN INDUSTRIAL BIOCATALYST. 2010 J. Mol. Catal B: Enzym. 62, 197–212.
  28. Angel Berenguer-Murcia and Roberto Fernandez-Lafuente *, 2010. New Trends in the Recycling of NAD(P)H for the Design of Sustainable Asymmetric Reductions Catalyzed by Dehydrogenases. Current Organic Chemistry 14, 1000-1021.
  29. Rafael C. Rodrigues, Roberto Fernandez-Lafuente*, 2010, LIPASE FROM Rhizomucor miehei AS AN INDUSTRIAL BIOCATALYST IN CHEMICAL PROCESSES. J. Mol. Catal B: Enzym 64, 1-22.
  30. Rafael C. Rodrigues, Benevides C. C. Pessela, Giandra Volpato, Roberto Fernandez-Lafuente, Jose M. Guisan and Marco A. Z. Ayub. 2010 Two step ethanolysis: a simple and efficient way to improve the enzymatic biodiesel synthesis catalyzed by an immobilized-stabilized lipase from Thermomyces lanuginosus Process Biochem. 45, 1268–1273.
  31. Rafael C. Rodrigues, Roberto Fernandez-Lafuente*, 2010. Lipase from Rhizomucor miehei as a biocatalyst in fats and oil modifications. J. Mol. Catal B: Enzym. 66, 15-32.
  32. Giandra Volpato, Rafael C. Rodrigues, Roberto Fernandez-Lafuente*. Use of enzymes in the production of semi-synthetic penicillins and cephalosporins: drawbacks and perspectives. Current Medicinal Chemistry; 2010; 17: 3855-73.
  33. Karel Hernandez and Roberto Fernandez-Lafuente*, 2011. CONTROL OF PROTEIN IMMOBILIZATION. Coupling immobilization and site directed mutagenesis to improve biocatalyst or biosensor performance Enzyme Microb. Technol. 48, 107-122.
  34. Karel Hernandez and Roberto Fernandez-Lafuente*, 2011, THE LIPASE B FROM Candida antarctica IMMOBILIZED ON OCTADECYL SEPABEADS: A VERY STABLE BIOCATALYST IN THE PRESENCE OF HYDROGEN PEROXIDE Process Biochem. 46 873-878.
  35. Giandra Volpato, Marco Filice, Blanca de las Rivas, Rafael C. Rodrigues, Júlio X. Heck, Roberto Fernandez-Lafuente, Jose M. Guisan, Cesar Mateo, and Marco Antônio Záchia Ayub Purification, chracterization and hyperactivation of a lipase from Staphylococcus warneri EX17 Biotechnol. Prog. 2011, 27, 717-723.


Profile Details: http://www.icp.csic.es/grupo-de-investigacion.php?id=24&lang=2


Last Updated: August, 2012


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