Prof. Roberto Fernández-Lafuente
Consejo Superior de Investigaciones Científicas, Spain
Professor
Email: rfl@icp.csic.es
Qualifications
1993-1996 Post doctorate, University Collegue London, United Kingdom
Publications (Selected)
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Gloria Fernandez-Lorente, Cesar Godoy, Adriano A. Mendes, Fernando Lopez-Gallego, Valeria Grazu, Blanca de las Rivas, Jose M. Palomo, Juan Hermoso, Roberto Fernandez-Lafuente* and Jose M. Guisan* 2008 SOLID-PHASE CHEMICAL AMINATION OF A LIPASE FROM Bacillus thermocatenulatus TO IMPROVE ITS STABILIZATION VIA COVALENT IMMOBILIZATION ON HIGHLY ACTIVATED GLYOXYL-AGAROSE. Biomacromolecules 9; 2553-2561.
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Marco Filice, Jose M Palomo, Paolo Bonomi, Teodora Bavaro, Roberto Fernandez-Lafuente, Jose M Guisan and Marco Terreni,2008 Preparation of linear oligosaccharides by a simple monoprotective chemo-enzymatic approach. Tetrahedron. 64, 9286-9292.
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Samia Kaddour, Fernando López-Gallego, Tahar Sadoun, Roberto Fernandez-Lafuente* and Jose M. Guisan*. (2008) PREPARATION OF AN IMMOBILIZED-STABILIZED CATALASE DERIVATIVE FROM Aspergillus niger HAVING ITS MULTIMERIC STRUCTURE STABILIZED: THE EFFECT OF Zn2+ON ENZYME STABILITY. J. Mol. Cat. B: Enzym. 55, 142–145.
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Adriano A. Mendes, Dasiana S. Rodrigues, Marco Filice, Roberto Fernandez-Lafuente, Jose M. Guisan and Jose M. Palomo. 2008. Regioselectivity alteration of lipases by immobilization in the monohydrolysis of per-O-acetylated 1-O-substituted-β-glucopyranosides. Tetrahedron 64, 10721-10727.
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K. Rose, S. Dzyadevych, R. Fernández-Lafuente, N. Jaffrezic, G. Kuncová, V. Matějec, P. Scully 2008 Hybrid Coatings as Transducers in Optical Biosensors J. Coating. Technol. Res. 5, 491 – 496.
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Cesar Carrasco-López, Cesar Godoy, Blanca de las Rivas, Gloria Fernandez-Lorente, Jose M. Palomo, José M. Guisán, Roberto Fernández-Lafuente, Martín Martínez-Ripoll and Juan A. Hermoso 2008 Crystallization of a bacterial thermoalkalophilic lipase BTL2 from Bacillus thermocatenolatus. Acta Cryst. F, 64, 1043–1045.
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Zaida Cabrera, Gloria Fernandez-Lorente, Jose M. Palomo, Jose M. Guisan*, Roberto Fernandez-Lafuente*. 2008 Asymmetric hydrolysis of dimethyl 3-phenylglutarate catalyzed by Lecitase Ultra®. Effect of the immobilization protocol on its catalytic properties. Enzyme Microb. Technol. 43, 531–536.
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Marco Filice, Teodora Bavaro, Roberto Fernandez-Lafuente, Massimo Pregnolato, Jose M Guisan, Jose M Palomo and Marco Terreni. 2009. Chemo-Biocatalytic Regioselective One Pot Synthesis of Different Deprotected Monosaccharides Catalysis today. 140, 11-18.
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Zaida Cabrera, Gloria Fernandez-Lorente, Roberto Fernandez-Lafuente, Jose M. Palomo and Jose M. Guisan. 2009 Enhancement of Novozym-435 catalytic properties by physical or chemical modification. Proc. Biochem. 44, 226-231.
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Juan M. Bolivar, Cesar Mateo, Javier Rocha-Martin, Felipe Cava, Jose Berenguer, Roberto Fernandez-Lafuente* and Jose M. Guisan*. 2009 THE ADSORPTION OF MULTIMERIC ENZYMES ON VERY LOWLY ACTIVATED SUPPORTS INVOLVES MORE ENZYME SUBUNITS: STABILIZATION OF GLUTAMATE DEHYDROGENASE FROM Thermus thermophilus BY IMMOBILIZATION ON HETEROFUNCTIONAL SUPPORTS.Enzyme Microb. Technol. 44, 139-144.
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Zaida Cabrera, Gloria Fernandez-Lorente, Roberto Fernandez-Lafuente, Jose M. Palomo and Jose M. Guisan. 2009, Novozym 435 displays very different selectivity compared toCandida antarctica Blipase adsorbed on standard hydrophobic supports J. Mol. Cat. B: Enzym. 57, 171-176.
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Aline G. Cunha, Gloria Fernández-Lorente, Melissa L. E. Gutarra, Juliana V. Bevilaqua, Rodrigo V. Almeida, Lúcia M. C. Paiva, Roberto Fernández-Lafuente, Jose M. Guisán, Denise M. G. Freire. 2009. Separation and immobilization of lipase from Penicillium simplicissimum by selective adsorption on hydrophobic supports Applied Biochemistry and Biotechnology, 156, 563–575.
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Dasciana S. Rodrigues, Adriano A. Mendes, Marco Filice, Roberto Fernandez-Lafuente, Jose M. Guisan and Jose M. Palomo. 2009 Different derivatives of a lipase display different regioselectivity in the monohydrolysis of per-O-acetylated 1-O-substituted-β-galactopyranosides. J. Mol. Cat. B: Enzym. 58, 36-40.
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Pilar Batalla, Cesar Mateo, Valeria Grazu, Roberto Fernandez-Lafuente* and Jose M. Guisan*. 2009 IMMOBILIZATION OF ANTIBODIES THROUGH THE SURFACE REGIONS HAVING THE HIGHEST DENSITY IN LYSINE GROUPS ON FINALLY INERT SUPPORT SURFACES. Proc. Biochem 44, 365-368.
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Juan M. Bolivar, Javier Rocha-Martin, Cesar Mateo, Felipe Cava, Jose Berenguer, Daniel Vega, Roberto Fernandez-Lafuente* and Jose M. Guisan*. 2009. Purification and stabilization of a glutamate dehygrogenase from Thermus thermophilus via oriented multisubunit plus multipoint covalent immobilization. J. Mol. Cat B: Enzym. 58, 158-163.
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César Carrasco-López, César Godoy, Blanca de las Rivas, Gloria Fernández-Lorente, José M. Palomo, José M. Guisán, Roberto Fernández-Lafuente, Martín Martínez-Ripoll, and Juan A. Hermoso 2009. Activation of bacterial thermoalkalophilic lipases is spurred by dramatic structural rearrangements J. Biol. Chem. 284, 4365-4372.
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César Carrasco-López, César Godoy, Blanca de las Rivas, Gloria Fernández-Lorente, José M. Palomo, José M. Guisán, Roberto Fernández-Lafuente, Martín Martínez-Ripoll, and Juan A. Hermoso 2009 Cubierta del numero 7 del volumen 284 de la revista J. Biol. Chem.
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Oscar Romero, José Vergara, Roberto Fernández-Lafuente, José Manuel Guisán, Andrés Illanes, Lorena Wilson. 2009. Simple strategy of reactivation of a partially inactivated penicillin acylase biocatalyst in organic solvent and its impact in the synthesis of β-lactam antibiotics. Biotechnol. Bioeng. 103, 472-479.
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Rafael C. Rodrigues, Juan M. Bolivar, Armand Palau-Ors, Giandra Volpato, Marco A. Z. Ayub, Roberto Fernandez-Lafuente* and Jose M. Guisan* 2009. Positive effects of the multipoint covalent immobilization in the reactivation of partially inactivated derivatives of lipasefrom Thermomyces lanuginosus. Enzyme Microb Technol. 44, 386-393.
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Rafael C. Rodrigues, Cesar A. Godoy, Marco Filice, Juan M. Bolivar, Armand Palau-Ors, Jesus M. Garcia-Vargas, Oscar Romero, Lorena Wilson, Marco A. Z. Ayub, Roberto Fernandez-Lafuente* and Jose M. Guisan*. 2009. Reactivation of covalently immobilized lipase from Thermomyces lanuginosus. Process Biochem . 44, 641-646.
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Juan M. Bolivar, Javier Rocha-Martin, Cesar Mateo, Felipe Cava,Jose Berenguer, Roberto Fernandez-Lafuente* and Jose M. Guisan*. 2009 COATING OF SOLUBLE AND IMMOBILIZED ENZYMES WITH IONIC POLYMERS: FULL STABILIZATION OF THE QUATERNARY STRUCTURE OF MULTIMERIC ENZYMES. Biomacromolecules. 10, 742–747.
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Juan M. Bolivar, Cesar Mateo, Cesar Godoy, Benevides C.C. Pessela, Dasciana S. Rodrigues,Raquel L. C. Giordano Roberto Fernandez-Lafuente* and Jose M. Guisan* 2009. The Co-operative Effect of Physical and Covalent Protein Adsorption on Heterofunctional Supports. Process. Biochem. 44, 757–763.
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Rafael C. Rodrigues, Cesar Godoy, Giandra Volpato, Marco A. Z. Ayub, Roberto Fernandez-Lafuente* and Jose M. Guisan*. 2009 Immobilization-stabilization of the lipase from Thermomyces lanuginosus: the critical role of the chemical amination. Process Biochem. 44, 963-968.
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Javier Rocha-Martin, Daniel E. Vega, Zaida Cabrera, Juan M. Bolivar, Roberto Fernandez-Lafuente*, Jose Berenguer* and Jose M. Guisan*2009. Purification, immobilization and stabilization of a highly enantioselective alcohol dehydrogenase from Thermus thermophilus HB27 cloned in E. coli. Process Biochem. 44, 1004-1012.
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Roberto Fernandez-Lafuente Stabilization of multimeric enzymes: strategies to prevent sub-unit dissociation 2009. Enzyme Microb. Technol. 45, 405–418.
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Rafael C. Rodrigues, Juan M. Bolivar, Giandra Volpato, Marco Filice, Cesar Godoy, Roberto Fernandez-Lafuente* and Jose M. Guisan* 2009. Improved reactivation of immobilized-stabilized lipase fromThermomyces lanuginosus by its coating with highly hydrophilic polymers. J. Biotechnol. 144, 113–119.
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Roberto Fernandez-Lafuente. LIPASE FROM Thermomyces lanuginosus. USES AND PROSPECTS AS AN INDUSTRIAL BIOCATALYST. 2010 J. Mol. Catal B: Enzym. 62, 197–212.
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Angel Berenguer-Murcia and Roberto Fernandez-Lafuente *, 2010. New Trends in the Recycling of NAD(P)H for the Design of Sustainable Asymmetric Reductions Catalyzed by Dehydrogenases. Current Organic Chemistry 14, 1000-1021.
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Rafael C. Rodrigues, Roberto Fernandez-Lafuente*, 2010, LIPASE FROM Rhizomucor miehei AS AN INDUSTRIAL BIOCATALYST IN CHEMICAL PROCESSES. J. Mol. Catal B: Enzym 64, 1-22.
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Rafael C. Rodrigues, Benevides C. C. Pessela, Giandra Volpato, Roberto Fernandez-Lafuente, Jose M. Guisan and Marco A. Z. Ayub. 2010 Two step ethanolysis: a simple and efficient way to improve the enzymatic biodiesel synthesis catalyzed by an immobilized-stabilized lipase from Thermomyces lanuginosus Process Biochem. 45, 1268–1273.
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Rafael C. Rodrigues, Roberto Fernandez-Lafuente*, 2010. Lipase from Rhizomucor miehei as a biocatalyst in fats and oil modifications. J. Mol. Catal B: Enzym. 66, 15-32.
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Giandra Volpato, Rafael C. Rodrigues, Roberto Fernandez-Lafuente*. Use of enzymes in the production of semi-synthetic penicillins and cephalosporins: drawbacks and perspectives. Current Medicinal Chemistry; 2010; 17: 3855-73.
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Karel Hernandez and Roberto Fernandez-Lafuente*, 2011. CONTROL OF PROTEIN IMMOBILIZATION. Coupling immobilization and site directed mutagenesis to improve biocatalyst or biosensor performance Enzyme Microb. Technol. 48, 107-122.
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Karel Hernandez and Roberto Fernandez-Lafuente*, 2011, THE LIPASE B FROM Candida antarctica IMMOBILIZED ON OCTADECYL SEPABEADS: A VERY STABLE BIOCATALYST IN THE PRESENCE OF HYDROGEN PEROXIDE Process Biochem. 46 873-878.
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Giandra Volpato, Marco Filice, Blanca de las Rivas, Rafael C. Rodrigues, Júlio X. Heck, Roberto Fernandez-Lafuente, Jose M. Guisan, Cesar Mateo, and Marco Antônio Záchia Ayub Purification, chracterization and hyperactivation of a lipase from Staphylococcus warneri EX17 Biotechnol. Prog. 2011, 27, 717-723.
Profile Details: http://www.icp.csic.es/grupo-de-investigacion.php?id=24&lang=2
Last Updated: August, 2012